Cell Growth, Differentiation and Senescence: A Practical ...

There are three main themes running through this completely updated edition of Cell Growth and Apoptosis. First, basic methods for measurement of cell proliferation are introduced and explained with reference to various systems, primarily in vitro, but with in vivo procedures also illustrated. The second theme is growth signalling, and is exemplified by methods for the analysis of transduction pathways for growth, beginning at the cell membrane and leading to the cell nucleus. The last theme presented here is growth cessation, illustrated by several systems for induction of celldifferentiation, and of cell senescence. The emphasis throughout the book is on human cell systems, making it particularly relevant to scientists interested in human disease, especially cancer. Many of the well proved methods for studying cell growth presented here are supplemented by some novelapproaches, e.g., studies of cell cycle checkpoints, cell spheroids, and nuclear architecture. The book is written by a team of scientists highly experienced in the procedures they describe, and they offer details and hints found valuable in their own laboratories. These should be helpful tobeginning as well as experienced investigators, and are designed to stimulate different approaches to old and new questions.