Biotechnology Resources
Biotechnology Resources As everyone knows, Dolan DNA Learning Center home page: http://www.dnalc.org/home.html. Sometimes called "molecular photocopying," this is the process used to amplify tiny amounts of DNA, producing accurate copies in ... [... more]
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Visualizing Molecules in Three Dimensions
Chemistry Guide to Visualizing Molecules in Three Dimensions Although we often see molecules depicted using two-dimensional (2-D) figures, they are in fact three-dimensional (3-D). Many scientific questions can be better understood by seeing and ... [... more]
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Molecular Scissors
In this experiment you will determine if cutting a piece of DNA with a restriction enzyme depends upon the sequence of the DNA. Once the DNA is cut apart, it can be put back together in different ways. For example, the gene for human insulin can ... [... more]
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What Makes a DNA Fingerprint Unique?
In this experiment you will test if unique DNA sequences can create individual fingerprints that are also unique. For example, a two base pair long DNA sequence can be one of sixteen different sequences: AA, AT, AC, AG, TA, TT, TC, TG, CA, CT, ... [... more]
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Table 2: Agglutination reaction pattern vs. blood type.
Identify the unknown "blood types" of the synthetic samples. Understand the importance of blood typing when transfusions are necessary for medical treatment. The human immune system has various ways of responding to an infection caused by ... [... more]
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Extracting Onion DNA
This procedure is designed to extract DNA from onion in sufficient quantity to be seen and spooled. The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology. The scientist must be able to ... [... more]
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A Juicy Project: Extracting Apple Juice with Pectinase
The goal of this project is to monitor enzyme activity by measuring the amount of apple juice released by pectinase. Enzymes are the workhorses of biochemistry. Enzymes are proteins that catalyze (speed up) specific chemical reactions-increasing ... [... more]
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Enzyme-Catalyzed Reactions -- What Affects Their Rates?
The goal of this project is to investigate enzyme kinetics, using catalase enzyme extracted from potatoes. Enzyme activity will be measured as a function of temperature. A protocol for measuring activity as a function of enzyme concentration is ... [... more]
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Building the Gel Electrophoresis Chamber
Gel Electrophoresis Science Project: Build a homemade gel electrophoresis chamber and use it to separate the components in food coloring dyes. In this science project you will build your own gel electrophoresis chamber and use it to compare ... [... more]
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Do-It-Yourself DNA
In this experiment, you will design a DNA Extraction Kit and use it to purify DNA from strawberries. Scientists study DNA for many reasons. They can figure out how the instructions stored in DNA help your body to function properly. They can use ... [... more]
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Liver Stinks!
In this experiment, you will extract the enzyme catalase from fresh liver, use it to break down hydrogen peroxide and test the activity of catalase under different conditions. A chemical reaction is when chemical come together and their molecules ... [... more]
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A Magnetic Primer Designer
In this experiment you will test how matches and mismatches affect the ability of primers to stick to the DNA that is copied during PCR. If these first three steps are repeated 30 times, a scientist can make 1 billion copies of a single piece of ... [... more]
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